| Information on Lab Supplies |
| Items we get from the stockroom |
| Commonly ordered lab supplies |
| Biochemicals we order |
| Supplies we order |
| Lab Pertinent Protocols |
| Digital Copy |
| General Research Web Tools |
| Buffer making |
| Protocol Online |
| Molecular Cloning Procedures |
| Agarose gel |
| Agarose gel extraction (Qiagen) (PDF) |
| Bacterial transformation |
| DNA sequencing (see website also) (Excel 2003 file) |
| DNA sequencing primer list |
| DNA sequencing result analysis |
| Ligation (Excel 2003 file) |
| Miniprep (Qiagen) (PDF) |
| MGM100 In Vivo Transformations (whole procedure overview) (Word file) |
| Molecular cloning (whole procedure overview) (Powerpoint 2007 file) |
| PCR |
| PCR purification (Qiagen) (PDF) |
| Primer design |
| Preparation of competent cells |
| Quik-Change site-directed mutagenesis |
| Restriction digest |
| TOPO reaction |
| Protein Expression and Purification Procedures |
| Overexpression test (small scale) |
| Large scale growth |
| Purification of GroEL (Word 2003 file) |
| Purification of SR1 (Word 2003 file) |
| Purification of GroES (single) (Word 2003 file) |
| Purification of 6His GroES7 (Word 2003 file) |
| General protein storage and stability (PDF) |
| Biochemistry Procedures |
| ATPase assay of GroEL and GroES (Word 2003 file) |
| MDH re-folding assay of GroEL and GroES (Word 2003 file) |
| FITC labeling of GroES (Word 2003 file) |
| Buffer Recipes |
| Recipe instructions for general buffers in the Chen lab |