IMPORTANT NOTE: Always check the sequence before you use it.
| Primer name | Sequence (5' to 3') |
| 5' GroEL seq primer | GGCAGCTAAAGACGTAAAATTCGG |
| 5' GroEL midseq primer | GGCGCAGTAGAACTGGAAAGCCCG |
| 3' GroEL seq primer | CCGCCCATGCCACCCATGCC |
| pCOLADuet MCS1 5' Also called: ACYCDuetUP1 |
GGATCTCGACGCTCTCCCT |
| pCOLADuet MCS1 3' Also called: DuetDOWN1 |
GATTATGCGGCCGTGTACAA |
| pCOLADuet MCS2 5' Also called: DuetUP2 |
TTGTACACGGCCGCATAATC |
| pCOLADuet MCS2 3' Also called: T7 terminator |
GCTAGTTATTGCTCAGCGG |
| pGEX 5' | GGGCTGGCAAGCCACGTTTGGTG |
| pGEX 3' | CCGGGAGCTGCATGTGTCAGAGG |
| pQe 5' | CCCGAAAAGTGCCACCTG |
| pQe 3' | TGAACAAATCCAGATGGAGTT |
| T7 promoter primer (5') | TAATACGACTCACTATAGGG |
| T7 terminator primer (3') | GCTAGTTATTGCTCAGCGG |
| GW1 (for TOPO vector) | GTTGCAACAAATTGATGAGCAATGC |
| GW2 (for TOPO vector) | GTTGCAACAAATTGATGAGCAATTA |